Part:BBa_K2685016
Degradation Part (ptet+Lip+LacI+plac+phi174E)
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This part is the best of the many degradation circuits we have built. It is made up of Lip and suicide switch.We cocultured BBa_K2685007 and BBa_K2685016 at different concentrations of tetracycline. Lip degradation effect was measured by using unit fluorescence intensity because Lip degraded tetracycline caused BBa_K2685007 concentration growth thereby increased its unit production fluorescence intensity. From the pictures we can see that the unit fluorescence intensity of BBa_K2685007 mixed with BBa_K2685016 is generally higher than that of BBa_K2685007 alone.This further indicates that Lip can efficiently degrade tetracycline in the environment.
The figure below shows the common validation of the different degradation circuits we built. Compared with the MNP and bi-enzyme degradation circuits, BBa_K2685016 is one of the parts with the best degradation effect.
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2355
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 565
Illegal AgeI site found at 542 - 1000COMPATIBLE WITH RFC[1000]
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